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KMID : 1020220150150061063
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Journal of Korean Society of Dental Hygiene
2015 Volume.15 No. 6 p.1063 ~ p.1071
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Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction
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Kim Young-Sun
Lee Young-Eun
Lee Jung-Hwa
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Abstract
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Objectives: The purpose of the study is to investigate the quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction.
Methods: Participants were voluntarily recruited at D university, and saliva samples were extracted before and after scaling. Multiple real-time polymerase chain reactions were used to analyze characteristics and the amount of nine kinds of periodontal pathogens; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, and Eikenella corrodens.
Results: After scaling, most periodontal pathogens except Eikenella corrodens were significantly decreased in all subjects(p<0.05). In addition, the percentage of microorganisms associated with disease, the microorganism risk index of periodontitis and the prevalence of red complex, orange complex, and Aggregatibacter actinomycetemcomitans was also significantly reduced after scaling(p<0.05).
Conclusions: Scaling decreased in the amount of major periodontal pathogens and periodontitis prevalence rate.
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KEYWORD
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periodontal pathogens, real-time polymerase chain reaction, scaling
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